ANA tests identify autoantibodies in the body. And, they are one of the tools that clinicians use to help diagnose lupus.
Rheumatologists review laboratory tests, symptom logs, and family history before making a lupus diagnosis. This can be a long and challenging process. ANA tests are a crucial element as they offer insight into the functioning of the immune system by measuring the presence of particular proteins in the blood.
The newest criteria for determining a lupus diagnosis was established by the American College of Rheumatology (ACR) and the European League Against Rheumatism (EULAR) in 2018. The first requirement of the criteria to be diagnosed is:
- Positive ANA test with a titer of at least 80
The titer value relates to the ratio of blood serum being tested to a dilution agent. So, a titer of 80 would mean that for every 80 parts of dilution, there was one part of blood serum. The higher the dilution, the more antibodies would need to be in the blood sample to return a positive result for the test.
8 thoughts on “ANA Tests and Lupus”
What if you have all the symptoms and a negative ANA? Your symptoms are relieved with Hydroxychloroqine.
Hello! According to the Johns Hopkins Lupus Center, about 2% of people with lupus will have a negative ANA. The diagnostic criteria does rely heavily on the ANA, but a negative ANA is possible and it may fluctuate over time.
You need the AVISE panel. John’s Hopkins also says the those with a negative ANA all have a positive Anti-SSA/Ro.
I was diagnosed with SLE thirty-five years ago at age thirty, although I had been symptomatic since age twelve. My lupus prep test came out positive. I then went to a rheumatologist and my ANA was negative but the double stranded anti-DNA test was positive. It remains positive to this day and recently I have been diagnosed with Lupus nephritis stage three. Anyone here have a similar experience? I have never had a positive ANA test result. Dietary and treatment suggestions other than steroids please. I have a psychotic reaction to steroids.
My ANA titter is 2560 (yes 2560). Chronic low RBC. High ESR. Severe joint inflammation. Last 3 years hematuria and Proteinuria (both unexplained and kidney infection without micro growth). Skin lesions. Hair loss. Fluid retention. Extreme fatigue. Among a list of other symptoms and flagged results worsening over last 3 years. First symptom 20 years ago (butterfly rash with photosensitive hives rash’s). And the Rheumatologist DX -Undifferentiated mixed tissue disease because she didn’t want to label me with Lupus yet! Why is it so difficult to get straight answers? Plaquenil helped at 3-4 months and now I’m getting worse again
I have had my ANA tested 3 times. 2 times it was 1:160 and 1 time negative. My double stranded anti-DNA is negative but my c4 was a 13 and ch50 a 33 . I have also tested positive for cardiolipin antibodies when he did blood work to ID it my IGM AB was at a 56.2 My sed rate has always been low at a 8.
I have no idea still what is going on with me, I’ve had severe fatigue along with joint and muscle pain for over 10 years I have tachycardia involvement with my heart that the cardiologist doesn’t know why. Multiple constant UTIs that lead into kidney infections in the blink of an eye I do have the butterfly rash it seems to show more on my face the worse I feel. I’m just lost and confused I’m so tired of not knowing what’s going on with me
i have a postive ANA 1:80 and negative in all the subtypes. I have other high inflammation markers that fluctuate alot like my CRP an HS-CRP, elevated D-dimer that fluctates, high CK that fluctuates, fatigue, joint pain, osteoarthritis that is creating bone spurs in many joints, headaches, random 99 fevers, red rashes in the sun especially on thighs. no one can figure out why my inflammation levels do this, i do not have a Lupus diagnosis…no one knows whats the issue..im 38. i wish i knew
ANA Ab screen POSITIVE
ANA Titer <1:40 ANA pattern none detected
DNA (ds) Ab, Crithidia IFA POSITIVE
DNA (AB) (DS) Crithidia Titer 1:40, Titer H (<1:10)
Rheumatoid factor 14
SM Antibody negative
SM/RNP Antibody negative
Sjogren’s Antibody SS-A 4.1 POSITIVE
Sjogren’s Antibody SS-B negative
I know I definitely will be diagnosed with Sjogren’s, my mouth is dry and peeling with inflamed gums.(can lupus cause the same mouth symptoms?). I have noticed last year when I was in the sun a lot I had an uncontrolled itchy rash on my arms. Can high platelets also indicate lupus? I appreciate any feedback on my current blood work. Do you think I might have lupus?